Specifications, Protocols, Methods
To better understand how the IGMM Instruments work we describe a little more about the modalities here. This may help decide which one is better for your experiments. We also detail our protocols, and information for publication.
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General
- See here for publication info and the CMVM policy regarding inclusion of scientific support staff in publications.
- Information about commonly used dyes and fluorescent proteins is here
- This is the Thermo Spectra-Viewer which is very useful for planning experiments
- The FSU Molecular Expressions page is a useful resource for more theory about Light Microscopy
Epifluorescence
- Technical specification of epifluorescent microscopes
- Detailed information about filtersets used on our systems
- Link to information about the different cameras available in the imaging facility
- Scaling information for the epifluorescent microscopes
Confocals:
- Technical specification of confocal microscopes
- Information about FRAP, FRET, FCS, and conditional Imaging, including manual for FRAP and FRET on Bumblebee
Super-resolution:
More information about the specification of the instruments can be found at the ESRIC website. Our ESRIC OMERO instance can be found here (under construction)
- Specification of the N-SIM and N-STORM and SORA systems
- Technical notes for N-SIM and N-STORM and original papers for the SORA
- Sample prep protocols for N-SIM and N-STORM (Nikon procedure), Alternative Easy Buffer Protocol, Test samples for SMLM
- A technical introduction reviewing SIM, open-source analysis though SIM-Tool box and the 2017 SIM Nature Protocols paper
- Link to ThunderSTORM and manual for it use in analysis of Single Molecule Localisation Microscopy data and a Github page for batch processing
- A useful method for quantifying single molecule localisation microscopy
- A list of very useful original papers which are helpful to read before starting out hosted on Nikon MicroscopyU
- More information about standard reagents for super-resolution microscopy
