Technical

To better understand how the IGC Instruments work we describe a little more about the modalities here. This may help decide which one is better for your experiments. We also detail our protocols, and information for publication.

Jump to…

• General
• Epifluoresence
• Confocals
• Super-resolution

General

• The AIR does ask for appropriate acknowledgement in publications. See here for publication/materials & methods info and the CMVM policy regarding inclusion of scientific support staff in publications the Royal Microscopial Society guidelines for citation of core facility staff in papers are here
• This is the Thermo Spectra-Viewer which is very useful for planning experiments. FPbase is also a great resource for information on fluorescent proteins and their spectra
• The FSU Molecular Expressions page is a useful resource for more theory about Light Microscopy

Epifluorescence

• Technical specification of epifluorescent microscopes
• Link to information about the different cameras available in the imaging facility
• Pixel Size Calculator-Use this to determine the pixel size used in your images and to set scale bars
• Restitching Axioscan images – Manual and link to Zen light to do this on your PC / laptop

Confocals:

• Technical specification of confocal microscopes
• Information about FRAP, FRET, FCS, and conditional Imaging, including manual for FRAP and FRET on Bumblebee

Super-resolution:

More information about the specification of the instruments can be found at the ESRIC website. Our ESRIC OMERO instance can be found here (under construction)

• Specification of the N-SIM and N-STORM and SORA systems
• Technical information for N-SIM and N-STORM and original papers for the SORA
• Sample prep protocols for N-SIM and SORA and N-STORM (Nikon procedure), Alternative Easy Buffer Protocol, Test samples for SMLM
• Manual for batch deconvolution of SORA data
• A technical introduction reviewing SIM, open-source analysis though SIM-Tool box and the 2017 SIM Nature Protocols paper
• Link to ThunderSTORM and manual for it use in analysis of Single Molecule Localisation Microscopy data and a Github page for batch processing
• A useful method for quantifying single molecule localisation microscopy
• A list of very useful original papers which are helpful to read before starting out hosted on Nikon MicroscopyU
• More information about standard reagents for super-resolution microscopy