High Content and Digital Pathology

Zeiss Axio Scan
  • Cost: £35 per hour
  • Location: CG06B – Back of room
  • Manual and SOP for Axioscan here Video here
  • Magnification: 2.5, 5, 10 x20 and x40
  • Fluorescent channels: DAPI, 488nm, 555 / 594, 647
  • Slide loader can hold up to 100 slides
  • Images can be viewed offline using Zen Lite or QuPath
  • Analysis – QuPath Youtube channel for how to videos here

The Axioscan is booked through PPMS. For those new to the PPMS system, please read our user manual which covers brightfield and fluorescence scanning techniques

Nanozoomer XR
  • Cost: £25 per hour
  • Location: ECRC Level 1 – Pathology labs
  • Manual and SOP for nanozoomer here
  • Magnification: x20 and x40 (plus different zoom images)
  • Channels: Colourimetric (H and E, DAB etc)
  • Slide loader can hold up to 320 slides.
  • Images can be viewed offline using NDP viewer, download here.
  • Analysis – QuPath Youtube channel for how to videos here

The Nanozoomer is booked through PPMS. For those new to the PPMS system, please read our user manual which explains how to register and make bookings. Contact Helen Caldwell for training and/or support

Olympus ScanR
  • Cost: £50 per hour
  • Location: CG06
  • Modality: Spinning disk confocal or Widefield
  • Fluorophores: DAPI, CFP, GFP, YFP, RFP, AF647 + BF + DIC (10-60x)
  • Objectives: 4x D, 10x D, 20x D, 30x S, 40x D, 40x O, 63x O
  • Software: ScanR
  • Applications: High-Content imaging Fixed/Live
  • Manual: ScanR
Scrumpy
  • Modality: Inverted Epifluorescence
  • Cost: £34 p/h
  • Room: CG18
  • Fluorophores: DAPI, CFP, GFP, YFP, RFP, AF647+BF
  • Objectives: 4x, 10x, 20x, 40x, 63x
  • Software: Nikon NIS-Elements
  • Application: Plate scanning, live imaging, 3D imaging, conditional imaging
  • Manual: ScrumpyScrumpy Video
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