Below are details of our epifluorescence systems, located in CG06B.
Macred & Limelight
- Upright Epifluorescence + Brightfield
- Cost: £34 p/h
- Fluorophores DAPI, GFP, RFP, TxRed, AF647, BF
- Objectives 5x, 10x, 20x, 40x, 63x, 100x
- Cameras: Hamamatsu Orca Flash 4.0 (16-bit)
- Software: Micromanager
- Manuals: Macred, Limelight
Britemac
- Upright Epifluorescence + Colour Brightfield
- Modality: Tiling & Stitching
- Cost: £34 p/h
- Fluorophores DAPI, GFP, RFP, Tex Red, AF647, BF, polarised light
- Objectives 2.5x, 5x, 10x, 20x, 40x (dry), 63x, 100x
- Cameras: Photometrics Prime BSI (FL; 16/12-bit) and Axiocam 305 (colour)
- Software: Zen 3.5
- Manual
Deconvolution System
- Modality Upright – Deconvolution
- Cost: £34 p/h
- Fluorophores DAPI, GFP, Tex Red, AF647, Aqua, Orange, Gold + BF/DIC
- Objectives 20x, 63x, 100x
- Camera: Photometrics Prime BSI (16/12-bit)
- Software: Zen 3.5
- Manual
Calvados & Scrumpy
- Modality: Inverted Epifluorescence
- Cost: £34 p/h
- Room: Scrumpy CG18, Calvados CG06B
- Transmitted contrast methods: Brightfield, Phase Contrast, DIC
- Fluorophores: DAPI, CFP, GFP, YFP, RFP, AF647+BF
- Objectives: 4x, 10x, 20x, 25x, 40x, 60/63x, 100x
- Cameras: Photometrics Prime BSI (FL; 12/16-bit) and Retiga R6 (colour; Calvados only)
- Software: Micromanager/NIS-Elements
- Application: Plate scanning, live imaging, 3D imaging, conditional imaging
- Manual: Calvados
- Manual: Scrumpy
IGC Advanced Imaging Resource 