Epifluorescence and Live Imaging

Below are listed our Epifluorescent microscopes, we also have a free of charge upright and inverted fluorescent microscope for checking samples. Both are located in CG06B. There are how to videos for using the hardware and how to alighn tran.

Macred, Limelight and Britemac
  • Upright Epifluorescence + Brightfield
  • Cost: £34 p/h
  • Room CG06B
  • Fluorophores DAPI, GFP, RFP, mCherry, AF647, BF
  • Objectives 2.5x, 5x, 10x, 20x, 40x, 63x, 100x
  • Software: Micromanager (Macred and Limelight), Zen 3.5 (Britemac)
  • Manual Britemac, Macred, Limelight
  • Modality Upright – Deconvolution
  • Cost: £34 p/h
  • Room CG06B
  • Fluorophores DAPI, GFP, RFP, AF647 +BF
  • Objectives 40x, 63x, 100x
  • Software: NIS-Elements
  • Manual Granny, Video
Calvados and Scrumpy
  • Modality: Inverted Epifluorescence
  • Cost: £34 p/h
  • Room: Scrumpy CG18, Calvados CG06B
  • Fluorophores: DAPI, CFP, GFP, YFP, RFP, AF647+BF
  • Objectives: 4x, 10x, 20x, 40x, 63x
  • Software: Micromanager/NIS-Elements
  • Application: Plate scanning, live imaging, 3D imaging, conditional imaging
  • Manual: Calvados, Calvados Video
  • Manual: Scrumpy, Scrumpy Video

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