Epifluorescence and Live Imaging

Below are details of our epifluorescence systems, located in CG06B.

Macred & Limelight
  • Upright Epifluorescence + Brightfield
  • Cost: £34 p/h
  • Fluorophores DAPI, GFP, RFP, TxRed, AF647, BF
  • Objectives 5x, 10x, 20x, 40x, 63x, 100x
  • Cameras: Hamamatsu Orca Flash 4.0 (16-bit)
  • Software: Micromanager
  • Manuals: Macred, Limelight
Britemac
  • Upright Epifluorescence + Colour Brightfield
  • Modality: Tiling & Stitching
  • Cost: £34 p/h
  • Fluorophores DAPI, GFP, RFP, Tex Red, AF647, BF, polarised light
  • Objectives 2.5x, 5x, 10x, 20x, 40x (dry), 63x, 100x
  • Cameras: Photometrics Prime BSI (FL; 16/12-bit) and Axiocam 305 (colour)
  • Software: Zen
  • Manual
Deconvolution System
  • Modality Upright – Deconvolution
  • Cost: £34 p/h
  • Fluorophores DAPI, GFP, Tex Red, AF647, Aqua, Orange, Gold + BF/DIC
  • Objectives 20x, 63x, 100x
  • Camera: Photometrics Prime BSI (16/12-bit)
  • Software: Zen
  • Manual
Calvados & Scrumpy
  • Modality: Inverted Epifluorescence
  • Cost: £34 p/h
  • Room: Scrumpy CG18, Calvados CG06B
  • Transmitted contrast methods: Brightfield, Phase Contrast, DIC
  • Fluorophores: DAPI, CFP, GFP, YFP, RFP, AF647+BF
  • Objectives: 4x, 10x, 20x, 25x, 40x, 60/63x, 100x
  • Cameras: Photometrics Prime BSI (FL; 12/16-bit) and Retiga R6 (colour; Calvados only)
  • Software: Micromanager/NIS-Elements
  • Application: Plate scanning, live imaging, 3D imaging, conditional imaging
  • Manual: Calvados
  • Manual: Scrumpy

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