Confocal

The imaging facility houses four confocals. We have detailed technical information about the instruments required for publication or further information. For long term or more complex work please contact the imaging team in advance. We have some videos for our IGC users about how to turn on the systems here:

Butterfly (Nikon A1R+) and Firefly (Nikon A1+)
  • Cost £50 p/h
  • Room CG20
  • Manual: Butterfly, Firefly, Video
  • Modality Point scanning confocal
  • Fluorophores DAPI, CFP, GFP, YFP, RFP, AF647 +BF
  • Objectives 4x, 10x, 20x, 40x, 63x, 100x
  • Software NIS Elements
  • Applications: Fixed, live, 3D and tile imaging. FRAP,
  • Offline viewing software: Here
Dragonfly (Andor)
  • Cost £50 p/h
  • Room: CG18
  • Manual: Here, Video,
  • Modality: Spinning disk confocal,
  • Fluorophores, DAPI, CFP, GFP, YFP, RFP, AF647, iRFP/AF700 + BF
  • Objectives 20x, 40x, 60x, 100x
  • Software Fusion / Imaris (offline viewing)
  • Applications: Live imaging, 3D imaging, large tile imaging, super-resolution imaging. Sample Chamber assembly video
  • How to open Dragonfly files in FIJI: Here
Leica Stellaris
  • Cost: £50 p/h
  • Room: CG06
  • Manuals: Confocal, FCS
  • Modality: Point scanning confocal
  • Fluorophores: DAPI, CFP, GFP, YFP, RFP, AF647 +BF
  • Objectives: 10x D, 20x D, 20x imm, 40x D, 40x W, 63x W, 63x O, 100x O
  • Software: LAS-X
  • Applications: Fixed, live, 3D and tile imaging, FCS Spectral unmixing, FLIM (TauSense), FRET & FRAP

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