Confocal

The imaging facility houses four confocals. We have detailed technical information about the instruments required for publication or further information. For long term or more complex work please contact the imaging team in advance. We have some videos for our IGMM users about how to turn on the systems here:

  Cost   £30 p/h    Name  Butterfly (Nikon A1R+)   Room  CG20   Manual:   Here    Modality  Point scanning confocal   Fluorophores  DAPI, CFP, GFP, YFP, RFP, AF647 +BF   Manufacturer  Nikon   Objectives  4x, 10x, 20x, 40x, 63x, 100x   Software  NIS Elements   Application  Fixed, live, 3D and tile imaging. FRAP, Conditional imaging (Nikon Jobs)   Offline viewing software:   Here    Technical specs for publications:   Here
Butterfly (Nikon A1R+) and Firefly (Nikon A1+)
  • Cost £30 p/h
  • Room CG20
  • Manual: Butterfly, Firefly
  • Modality Point scanning confocal
  • Fluorophores DAPI, CFP, GFP, YFP, RFP, AF647 +BF
  • Objectives 4x, 10x, 20x, 40x, 63x, 100x
  • Software NIS Elements
  • Application Fixed, live, 3D and tile imaging. FRAP,
  • Offline viewing software: Here

  Cost   £30 p/h    Name  Dragonfly   Room  CG18   Manual   Here    Modality  Spinning disk confocal   Fluorophores,  DAPI, CFP, GFP, YFP, RFP, AF647, iRFP/AF700 + BF   Manufacturer  Andor   Objectives  20x, 40x, 60x, 100x   Software  Fusion   Application  Live imaging, 3D imaging, large tile imaging     Offline viewing software:   Here    Technical specs for publications:   Here
Dragonfly (Andor)
  • Cost £30 p/h
  • Room: CG18
  • Manual: Here
  • Modality: Spinning disk confocal,
  • Fluorophores, DAPI, CFP, GFP, YFP, RFP, AF647, iRFP/AF700 + BF
  • Objectives 20x, 40x, 60x, 100x
  • Software Fusion / Imaris (offline viewing)
  • Application Live imaging, 3D imaging, large tile imaging, super-resolution imaging
  • How to open Dragonfly files in FIJI: Here
  Cost   £30 p/h    Name  Bumblebee (Leica SP5)   Room  CG13   Manuals:       Confocal  or  FCS    Modality  Point scanning confocal   Fluorophores  DAPI, CFP, GFP, YFP, RFP, AF647 +BF   Manufacturer  Leica   Objectives  4x, 10x, 20x, 40x, 63x   Software  LAS-AF   Application  Fixed, live, 3D and tile imaging, FCS  Spectral unmixing, FRET + FRAP   Offline viewing software:   Here    Technical specs for publications:   Here
Bumblebee (Leica SP5)
  • Cost: £30 p/h
  • Room: CG13
  • Manuals: Confocal, FCS
  • Modality: Point scanning confocal
  • Fluorophores: DAPI, CFP, GFP, YFP, RFP, AF647 +BF
  • Objectives: 4x, 10x, 20x, 40x, 63x
  • Software: LAS-AF
  • Application Fixed, live, 3D and tile imaging, FCS Spectral unmixing, FRET + FRAP

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