We have a facility YouTube channel where we host how-to videos. Follow our channel or check out the videos embedded below. We also save playlists created by other people or organisations that you may find useful.
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Setting scale and adding a scale bar in ImageJ.
The document with the scaling relevant to the IGMM AIR microscopes can be found on the Technical Specifications Page.
Changing channel colours in ImageJ.
Watch this video to find out about changing lookup tables for your multi-channel images in ImageJ.
Splitting and merging channels in ImageJ
Introduction to the channels tool in ImageJ.
This useful tool can be used to easily switch between viewing your channels separately or together.
How to show images with the same display values
How to propogate the same minimum and maximum values to a series of images to compare your conditions.
Making a simple montage in ImageJ
Creating a intensity profile plot in ImageJ.
The plot profile tool in ImageJ can be used to create a plot of intensity values across features in your image.
One of the macros created in the facility will take your Micro-Manager images and save them as RGB for display. This video demonstrates how to use that macro which can be found on our Github.
Making a simple movie in Imaris
How to setup μManager
Most of the epifluoresence microscopes use μManager for acquisition. This video shows you to initially configure μManager the first time you log on to a new microscope.
Tiling with μManager
How to set up a tiling acquisition and then stitch the image once acquisition is complete.